Relative expression of fecal CK19 from younger patients and age-matched healthy controls. The levels of fecal CK19 were quantified by quantitative real-time PCR from who were under the age of 60, including 57 healthy controls, 36 patients with M0 stage, and 9 patients with M1 stage. The relative expression of fecal CK19 mRNA was normalized by dividing it by that for 18s rRNA for each fecal sample. A significant difference (p < 0.05) determined by Dunn's multiple comparison was directly indicated as p value. The horizontal lines represent the median values.