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Figure 2 | BMC Cancer

Figure 2

From: Identification of tyrosine-phosphorylated proteins associated with metastasis and functional analysis of FER in human hepatocellular carcinoma cells

Figure 2

Scheme of enrichment and identification of pTyr proteins. Cells were firstly lysed in modified RIPA buffer. pTyr proteins and their interacting partners were then immunoprecipitated by anti-pTyr antibodies. Eluted proteins were further digested into peptides and used to IMAC for second phosphopeptides enrichment. The sample was lastly analyzed by LC-MS/MS.

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