Figure 2From: Identification of tyrosine-phosphorylated proteins associated with metastasis and functional analysis of FER in human hepatocellular carcinoma cells Scheme of enrichment and identification of pTyr proteins. Cells were firstly lysed in modified RIPA buffer. pTyr proteins and their interacting partners were then immunoprecipitated by anti-pTyr antibodies. Eluted proteins were further digested into peptides and used to IMAC for second phosphopeptides enrichment. The sample was lastly analyzed by LC-MS/MS.Back to article page