Figure 6

The two ST3Gal.I transcripts are expressed in the majority of the bladder cancer and urothelium cells. A: Schematic diagram of human ST3Gal.I cDNA (on the top) and genomic (on the centre) sequences. The black rectangle in ST3Gal.I cDNA represents the sequence which is present in ST3Gal.I transcript variant NM_003033.2 and absent on the transcript variant NM_173344.2. Exons containing the coding region (represented in grey) are denoted as 1-6 with the sizes shown above. The total of exon 1 nucleotide sequence is shown below: DNA sequence absent in transcript variant NM_173344.2 is represented on black background and the location of the primers F3 and R1 used to discriminate the transcript variants is underlined. B: RT-PCR analysis of NM_003033.2 and NM_173344.2ST3Gal.I transcripts in bladder cancer cell lines and patient's specimens. The ST3Gal.I exon 1 was amplified in cDNAs obtained from HT1374, MCR, T24 and 5637 bladder cancer cell lines and bladder tumor (T) tissue and corresponding normal urothelium (U) from 6 randomly chosen patients (subgrouped as described above), using F3 and R1 primers. The predicted sizes of the products are 609 bp (NM_003033.2) and 406 bp (NM_173344.2).