Figure 5

Effects of celastrol on GA-induced activation of NF-kappa B. a: Nuclear translocation of the p65 subunit of NF-kappa B in Tca8113 cells after 12 hours of treatment with 4 μM GA and 0.5 μM celastrol (A: control, without nuclear staining, B: control, with nuclear staining, C: 12 hours after treatment with 4 μM GA alone, without nuclear staining, D: 12 hours after treatment with 4 μM GA alone, with nuclear staining, E: 12 hours after combined treatment with 4 μM GA and 0.5 μM celastrol, without nuclear staining, F: 12 hours after combined treatment with 4 μM GA and 0.5 μM celastrol, with nuclear staining). b: Nuclear translocation of the p65 subunit of NF-kappa B in Tca8113 cells after a 24 hours transient transfection of the dominant mutant pBabe-SR-IκBα and 12 hours treatment with 4 μM GA. (A: control, without nuclear staining, B: control, with nuclear staining, C: 24 hours transfection with pBαbe and 12 hours treatment with 4 μM GA, without nuclear staining, D: 24 hours transfection with pBabe and 12 hours treatment with 4 μM GA, with nuclear staining, E: 24 hours transfection with pBabe-SR-IκBα and 12 hours treatment with 4 μM GA, without nuclear staining, F: 24 hours transfection with pBabe-SR-IκBα and 12 hours treatment with 4 μM GA, with nuclear staining). c: EMSA analysis of NF-kappa B binding activity in Tca8113 cells after 12 hours of treatment with 4 μM GA and 0.5 μM celastrol (A: control, B: after treatment with GA alone, C: after combined treatment with GA and celastrol).