Akt phosphorylation in FHCC98 and 7721 cells. Forty-eight hours after siCD147 or sncRNA transfection of FHCC98 and 7721 cells, phospho-Akt and total-Akt expression levels were examined by Western blot. Bars represent the mean of triplicate samples; error bars represent standard deviation. Data are representative of three independent experiments. ** P < 0.01 versus corresponding controls.