Table 2 Details of primary antibodies
Target | Clone | Supplier | DAB concentration | Antigen retrieval | |
|---|---|---|---|---|---|
ERα | mouse | 6F-11 | Novocastra | 1:120 | 0.01 M Citrate buffer (pH6) for 5 minutes |
ERβ1 | mouse | PPG5/10 | Serotec, [60] | 1:40 | 0.05 M Glycine/EDTA (pH8) for 5 minutes |
ERβ2 | mouse | 57/3 | Serotec, [38] | 1:30 | 0.05 M Glycine/EDTA (pH8) for 5 minutes |
ERβ5 | mouse | 5/25 | Serotec, [61] | 1:75 | 0.01 M Citrate buffer (pH6) for 5 minutes |
PR (A+B) | rabbit | C19 | Santa Cruz | N/A | 0.01 M Citrate (pH6) 10 min |
COX-2 | goat | C20 | Santa Cruz | N/A | 0.01 M Citrate (pH6) 10 min |