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Table 2 Details of primary antibodies

From: Expression of oestrogen receptors, ERα, ERβ, and ERβ variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERα

Target   Clone Supplier DAB concentration Antigen retrieval
ERα mouse 6F-11 Novocastra 1:120 0.01 M Citrate buffer (pH6) for 5 minutes
ERβ1 mouse PPG5/10 Serotec, [60] 1:40 0.05 M Glycine/EDTA (pH8) for 5 minutes
ERβ2 mouse 57/3 Serotec, [38] 1:30 0.05 M Glycine/EDTA (pH8) for 5 minutes
ERβ5 mouse 5/25 Serotec, [61] 1:75 0.01 M Citrate buffer (pH6) for 5 minutes
PR (A+B) rabbit C19 Santa Cruz N/A 0.01 M Citrate
(pH6) 10 min
COX-2 goat C20 Santa Cruz N/A 0.01 M Citrate
(pH6) 10 min
  1. **Details of secondary antibodies are given in Table 4.