Ectopic expression of Par3 protein in the LNCaP cells. (A). Western blotting with anti-Flag (left) or with anti-PARD3 (right) antibody shows PARD3 expression in whole-cell lysates from the LNCaP cells stably transfected with Flag-tagged PARD3 expression vector but not in control nontransfected cells or transfected with EGFP empty vector. Blotting with anti-β-actin antibody was used as a loading control. (B) Immunofluorescent analysis with anti-flag antibodies shows localization of exogenous Flag-tagged Par3 protein at cell-cell contacts. Size bar is 20 μm.