Table 2 Primer sequences and annealing temperatures used in this study
Sequence | Annealing temperature (°C) | |
|---|---|---|
Semiquantitative Realtime PCR (Light Cycler) | ||
GLI1 | Forward: 5'- AGGGAGTGCAGCCAATACAG -3' Reverse: 5'- ATTGGCCGGAGTTGATGTAG -3' | 65 |
GAPDH | Forward: 5'- GAAGGTGAAGGTCGGAGTCA -3' Reverse: 5'- AATGAAGGGGTCATTGATGG -3' | 65 |