Figure 5

Lymphocyte CD25 expression and viability (7-AAD ^low ) after macrophage co-culture. (A) Lymphocytes co-cultured with macrophages for 24 h (treated or untreated) without cell contact (0.4 μm insert). CD25 expression and viability significantly increased after treatment. In Ly/Mg* was observed a higher amount of unviable (7-AAD⁺) Ly cells in control group. Unviable cells were not included in CD25 analysis for to keep comparison between viable CD25+ cells. (B) Melanoma cells co-cultured with lymphocytes previously co-cultured with macrophages. Control lymphocyte CD25 expression and viability significantly decreased after 24 h. Y-axis: transformed mean.