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Figure 4 | BMC Cancer

Figure 4

From: The cyclin-dependent kinase inhibitor 5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole induces nongenotoxic, DNA replication-independent apoptosis of normal and leukemic cells, regardless of their p53 status

Figure 4

DRB-induced apoptosis is independent of ATM and NBS1. (A) Immunoblot analysis of p53 and p53pSer15 in AT and NBS cells. Time course analysis of whole cell lysates of cultured T cells from one control, one AT and one NBS patient harvested before (0) or after either 2 Gy γ-IR or 100 μM DRB treatment. One representative experiment out of three is shown. (B, C) Confocal analysis of p53 and Bax localization. Cultured T lymphocytes from one AT and one NBS patient were harvested before or 6 hr after 100 μM DRB and stained for either p53 or Bax with green MitoTraker as cytsolic marker. One representative experiment out of two is shown. (D) AT and NBS cell survival and caspase activation following DRB treatment. Left panel: cultured T cells from one AT, one NBS patient and four controls were kept in medium alone or containing 100 μM DRB, harvested at 24, 48 and 72 hr and analyzed by flow cytometry after staining with PI. Right panel: cultured T cells from one AT (black bars) and one NBS (white bars) patient were kept for 24 hr in medium alone or containing different concentrations of DRB, then harvested and stained with anti-active caspase-3 antibody. Histograms show the percentage of active caspase-3+ cells.

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BMC Cancer

ISSN: 1471-2407

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