Figure 5
Electrophoretic gel mobility shift assays. GST-AP-2γ fusion protein was incubated with 32P-labeled wild-type or mutated oligonucleotides (marked by asterisk). The protein-DNA bindings were also confirmed by competition experiments with unlabeled AP-2γ consensus binding sequence in human metallothionein IIa promoter (HMT). The retarded bands showed positive binding results, and the intense bands at the bottom were unbound labeled probes. Data confirmed the binding elements on HPSE, CDH2 and IGSF11.