cDNA sequencing improves the detection of P53 missense mutations in colorectal cancer

BMC Cancer

Table 1 Results of molecular and immunohistochemical analyses of P53 gene.

No	17 p	Mutation	Exon	Codon	Effect	cDNA	DNA	Cells without LOH 17 p	Normalized relative expression level of *P53*mRNA
1	LOH	CGT>CAT	8	273	Arg→His	WT = MT	WT = MT	25–35%	3,201
2	LOH	GTG>ATG	5	173	Val→Met	only MT	MT>WT	15–25%	2,934
3	LOH	CGG>TGG	7	248	Arg→Trp	only MT	WT	50–60%	3,796
4	LOH	CGC>CAC	5	175	Arg→His	only MT	WT = MT	25–35%	4,235
5	LOH	CGT>CAT	8	273	Arg→His	MT>WT	WT = MT	25–35%	3,497
6	LOH	GAG>AAG	8	285	Glu→Lys	MT>WT	WT	40–50%	4,443
7	U	GGC>AGC	7	245	Gly→Ser	only MT	WT>MT	-	3,005
8	ROH	CGG>CAG	7	248	Arg→Gln	only MT	WT>MT	-	3,928
9	ROH	CGT>CAT	8	273	Arg→His	MT>WT	WT>MT	-	1,692
10	LOH	CGG>TGG	8	282	Arg→Trp	only MT	WT>MT	25–35%	3,307
11	ROH	GGC>AGC	7	245	Gly→Ser	MT = WT	WT>MT	-	2,213
12	NA	ATG>ATA	7	237	Met→Ile	only MT	NA	-	4,160
13	LOH	GTG>ATG	6	216	Val→Met	MT>WT	WT = MT	25–35%	2,478
14	LOH	GGC>AGC	7	245	Gly→Ser	MT = WT	WT>MT	35–45%	3,271
15	LOH	CGC>CAC	5	175	Arg→His	only MT	WT = MT	25–35%	4,215
16	LOH	negative						15–25%	2,607
17	LOH	negative						25–35%	2,932
18	LOH	negative						15–25%	2,606
19	LOH	13372 ins C	6	205	Frameshift	WT = MT	WT = MT	50–60%	1,803
20	ROH	negative						-	4,017
21	LOH	negative						15–25%	2,981
22	LOH	negative						15–25%	1,867
23	LOH	negative						25–35%	1,482

LOH, loss of heterozygosity; MT, mutated template; MT>WT, prevalence of mutated template; NA, not analyzed; WT, wild type template; WT = MT, equal amount of wild type and mutated template; WT>MT, prevalence of wild type template; ROH, retention of heterozygosity; U, uninformative (samples 1–18, positive immunohistochemistry; samples 19–23, negative immunohistochemistry).

ISSN: 1471-2407