Figure 1

Molecular analyses of P53 gene. (A) cDNA sequencing showing mutation (arrow) and DNA analysis confirming presence of wild type nucleotide only (case no. 3 in Table 1). (B) cDNA and DNA sequencing showing both bands equally (case no. 1 in Table 1). (C) cDNA sequencing showing prelevance of mutated nucleotide, and DNA presenting prelevance of wild type band (case no. 9 in Table 1). (D) estimation of sequencing sensitivity for sample presenting only mutated band, see Materials and Methods (case no. 3 in Table 1). (E) example of LOH analysis for microsatellite marker D17S675. The lost allele is marked with an arrow (case no.13, left and 10, right in Table 1). LOH analysis confirms a high amount of nontumor cells (cells without LOH on 17 p) in sample no. 13. N, normal tissue (blood); T, tumor sample.