Figure 3From: The critical role of ERK in death resistance and invasiveness of hypoxia-selected glioblastoma cells Determination of Ras activity and ROS level in HRT98G cells. (A) The Ras activity was assessed by precipitating GTP-bound active Ras with Raf-1 Ras binding domain-agarose conjugate (Top panel). The lower panel demonstrates that equal amounts of Ras were present. (B) The levels of ROS was measured in T98G and HRT98G using DCFH-DA ROS-sensitive fluorescence dye.Back to article page