Glioblastoma cells were cultured for 24 h before the addition of JAI-51 (10 μM) for 48 h. Caspase-3 activity, the percentage of cells with a DNA content <2 N (hypodiploid cells) and the percentage of apoptotic and necrotic cells were assessed. Results are the mean ± SD of 3 experiments. In the control cultures (not treated with JAI-51), Caspase activity was 4 ± 1 A.U. and the percentage of apoptotic cells was < 3% in all the cell lines. A.U.: arbitrary units; %: percentage of cells. * P < 0.05 (treated versus control).