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Table 2 Effect of JAI-51 on the apoptotic process in the glioblastoma cell lines.

From: A novel chalcone derivative which acts as a microtubule depolymerising agent and an inhibitor of P-gp and BCRP in in-vitro and in-vivoglioblastoma models

Cell line U118 U138 U373 LN229 GL26
Caspase-3 activity (A.U.) 13 ± 3* 6 ± 2 39 ± 3* 120 ± 25* ND
Hypodiploid cells (%) 26 ± 10* 17 ± 1* 20 ± 2* 30 ± 5* 25 ± 5*
Apoptotic+necrotic cells (%) 21 ± 5* 20 ± 3* 34 ± 2* 31 ± 2* 25 ± 8*
  1. Glioblastoma cells were cultured for 24 h before the addition of JAI-51 (10 μM) for 48 h. Caspase-3 activity, the percentage of cells with a DNA content <2 N (hypodiploid cells) and the percentage of apoptotic and necrotic cells were assessed. Results are the mean ± SD of 3 experiments. In the control cultures (not treated with JAI-51), Caspase activity was 4 ± 1 A.U. and the percentage of apoptotic cells was < 3% in all the cell lines. A.U.: arbitrary units; %: percentage of cells. * P < 0.05 (treated versus control).