Table 1 Primer-sequences of primers used in this report.
From: Regulation of MYCNexpression in human neuroblastoma cells
Gene of interest | Genbank ID | Forward primer | Reverse primer |
|---|---|---|---|
QPCR | |||
MYCN | NM_005378.4 | 5'-cacaaggccctcagtacctc-3' | 5'-accacgtcgatttcttcctc-3' |
ΔMYCN | Not present | 5'-cagaatcgcctccggatc-3' | 5'-cgcttctccacagtgacca-3' |
MYCNOS | S49953.1 | 5'-tccgacagctcaaacacagac-3' | 5'-ccagctttgcagccttctc-3' |
MYCN total | NM_005378.4 | 5'-cataaggggtttgccatttg-3' | 5'-ctaatactggccgcaaaagc-3' |
GUSB | NM_000181.1 | 5'-agagtggtgctgaggattgg-3' | 5'-ccctcatgctctagcgtgtc-3' |
TFRC | NM_003234.1 | 5'-gttcttctgtgtggcagttcag-3' | 5'-caggctgaaccgggtatatg-3' |
RNFIII | NM_017610.6 | 5'-gcagaatgcagcagaagttg-3' | 5'-ccattcttgcagaagtggttg-3' |
gQPCR | |||
MYCN (exon 1) | NM_005378.4 | 5'-ccgggtgtgtcagatttttc-3' | 5'-tccaacacagttcccaggag-3' |
MYCN (exon 2) | NM_005378.4 | 5'-gatctgcaagaacccagacc-3' | 5'-ccgccgaagtagaagtcatc-3' |
MYCN (exon 3) | NM_005378.4 | 5'-gttcctcctccaacaccaag-3' | 5'-aggcatcgtttgaggatcag-3' |
MYCN (3'-UTR) | NM_005378.4 | 5'-taccaggtgcaggagagacc-3' | 5'-agcccaagtagccaagacac-3' |
MYCNOS | S49953.1 | 5'-aagaagggtagtccgaaggtg-3' | 5'-gaaactggaaacatccagagg-3' |
CFTR | NM_000492.3 | 5'-gggtcttgataaatggcttcc-3' | 5'-tctggcttgcaaaacacaag-3' |
TBX22 | NM_016954.2 | 5'-tttaccggctcctgaaagac-3' | 5'-aaccgctttttgaattggtg-3' |
SLC16A2 | NM_006517.2 | 5'-cttcttcgtccctctgatgc-3' | 5'-tcaggggccaacatcttatc-3' |
Cloning | |||
MYCNOS | S49953.1 | 5'-agggggtggtggcgaggc-3' | 5'-gtagctcgcacttatttatttat-3' |