RCC10 cells with type 1, but not type 2 mutant VHL proteins lack an epithelial-like morphology. (A) Indicated RCC10 cell lines were grown to confluence on collagen I and photographed by digital phase microscopy (original magnification of 100×). Note the disorganized morphology of the control and the VHL del 114–178 and RC161/2QW-expressing cells. (B) A498 cell lines were similarly analyzed by phase microscopy. Note that all of the A498 cell lines display a disorganized non-epithelial phenotype.