Figure 7
Silencing SFRP5 by RNAi in MKN1 cells. (A) SFRP5 mRNA was not detected in MKN1 after SFRP5 RNAi treatment by RT-PCR. (B) SFRP5 protein was not detected in MKN1 after SFRP5 RNAi treatment by Western blotting. (C) Real-time PCR analysis showed MMP-7 mRNA and MT1-MMP mRNA were upregulated in MKN1 after transfection with SFRP5 RNAi plamid. Data are expressed as mean ± SD, n = 3, *P < 0.001, vs Control RNAi. (D) Weatern blotting analysis showed MMP-7 protein and MT1-MMP protein were upregulated in MKN1 after transfection with SFRP5 RNAi plamid.