Figure 4

Collapse of mitochondrial ΔΨm induced by Res. U251 cells were cultured and incubated with Res (150 μM) for 6–72 h. Cells were stained with JC-1 and examined with fluorescence microscopy. Cells with normal mitochondria predominantly show red fluorescence. Cells with decreased mitochondrial membrane potential emit diffuse green fluorescence in the cytoplasm. Microphotographs were shown as representative results from three independent experiments. ×400.