Figure 4
From: KIF11 inhibition for glioblastoma treatment: reason to hope or a struggle with the brain?
Cell cycle profile on U87MG GBM cells. Effect of the compound 1 on the cell cycle of U87MG cells. The cells were un-treated (figure 4a), treated with 1 μM of the Ispinesib analog compound 1 for 24 hours (Figure 4b) after 24 hours starvation. Nocodazole treatment was used as positive control (Figure 4c). Following incubation, the DNA of the cells was stained with propidium iodide and its content was analyzed by flow cytometry. Compound 1 induced a block of the cell cycle in G2/M phase. M1 was marker 1 and it represented G0/G1 phase; M2 was marker 2 and it represented the S phase; M3 was marker 3 and it represented G2/M phase.