Figure 2
From: Systematic validation of predicted microRNAs for cyclin D1
Expression of exogenous microRNAs used for validation. A, Schematic description of microRNA constructs. B, Expression of three microRNAs that were identified to be positive in this study. 293T cells were first transfected with indicated miRNAs and harvested 24 h after infection for extraction of RNA. The relative expression was determined by real-time PCR. Values are means of three transfection experiments.