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Table 3 Antibodies for Western blot and immunohistochemistry

From: Expression of matrix metalloproteinases (MMPs) in primary human breast cancer and breast cancer cell lines: New findings and review of the literature

Gene Application Protein forms detected by WB Species Type/clone Dilution In WB Dilution in IHC-P and ICC Company
MMP-1 WB, IHC latent and active rabbit polyclonal 1:750 1:100 Biozol
MMP-2 WB, IHC latent and active rabbit polyclonal 1: 1000 1:100 Abcam
MMP-3 WB, IHC, ICC latent and active mouse SPM 293 1: 500 1:50 Abcam
MMP-7 WB latent and active mouse 111433 1: 500 - Abcam
MMP-8 WB latent and active mouse 115-13D2 1: 1000 - Chemicon
MMP-9 WB latent and active mouse 9D4.2 1: 500 - Chemicon
MMP-10 WB, IHC, ICC latent and active mouse IVC5 1: 500 1:100 Chemicon
MMP-11 WB latent and active mouse SL 3.01 1: 500 - Abcam
MMP-11 IHC, ICC latent and active mouse SPM 199 - Prediluted Biozol
MMP-12 WB latent and active rabbit polyclonal 1: 1000 - Abcam
MMP-13 WB, IHC latent and active mouse 87512 1: 500 1:100 R&D
MMP-14 WB, IHC, ICC latent and active rabbit polyclonal 1: 500 1:75 Abcam
MMP-15 WB, IHC, ICC latent and active rabbit polyclonal 1: 500 1:100 Abcam
MMP-19 WB latent and active rabbit polyclonal 1: 3000 - Biozol
MMP-19 IHC, ICC latent and active rabbit polyclonal - Prediluted Biozol
MMP-23 WB latent and active rabbit polyclonal 1: 1000 - Abcam
MMP-24 WB latent and active rabbit polyclonal 1: 1000 - Abcam
MMP-27 WB not specified rabbit polyclonal 1: 1000 - Abcam
MMP-28 WB not specified rabbit polyclonal 1: 1000 - Abcam
β-actin WB b-actin mouse M/Abcam 8226 1: 10.000 - Abcam
  1. WB: Western blot; IHC-P: Immunohistochemistry (formalin-fixed paraffin-embedded section),
  2. ICC: Immunocytochemistry