Figure 6

Expression of MMPs in different human breast cancer cell lines analyzed by semiquantitative RT-PCR. Total RNA from breast cancer cell lines MCF-7, MDA-MB-468, BT 20 and ZR 75/1 was extracted and used as template for RT-PCR analysis. Primers, specific for each transcript, were designed in flanking exons (see Additional file 2: primer sequences), resulting in longer amplicons if human genomic DNA was amplified (positive control (+)) and in shorter amplicons representing cDNAs. The gene PBGD was used as internal loading control and amounts of cDNA were normalized to the amount of PBGD for each sample.