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Table 6 Comparison of automated IHC scores with visual scores and FISH results

From: Inter-observer reproducibility of HER2 immunohistochemical assessment and concordance with fluorescent in situhybridization (FISH): pathologist assessment compared to quantitative image analysis

IHC/FISH

Machine 1

Machine 2

Visual 1

Visual 2

FISH

Negative (IHC 0,1+/FISH <1.8)

434

445

415

431

394

Equivocal (IHC 2+/FISH 1.8–2.2)

105

163

52

48

37

Positive (IHC 3+/FISH >2.2)

77

8

149

137

185