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Figure 2 | BMC Cancer

Figure 2

From: Combining the receptor tyrosine kinase inhibitor AEE788 and the mammalian target of rapamycin (mTOR) inhibitor RAD001 strongly inhibits adhesion and growth of renal cell carcinoma cells

Figure 2

Adhesion of RCC cells to HUVEC. A498, Caki-1 or KTC-26 cells were treated with 1 μM AEE788 or 1 nM RAD001, applied alone or in combination. After a 1 h pre-incubation, tumor cells were added at a density of 0.5 × 106 cells/well to HUVEC monolayers for 60 min. Non-adherent tumor cells were washed off in each sample, the remaining cells were fixed and counted in five different fields (5 × 0.25 mm2) using a phase contrast microscope. Mean values were calculated from five counts. Mean adhesion capacity is depicted as adherent cells/mm2. One representative of six experiments is shown. * indicates significant difference to controls (p < 0.05).

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BMC Cancer

ISSN: 1471-2407

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