Figure 5
From: Identification of hematein as a novel inhibitor of protein kinase CK2 from a natural product library
Inhibition effects of hematein on cellular growth in normal and cancer cells. A. Normal (WI-38, CCL-211) and cancer (Hela, A549 and A427, HCT116) cells were cultured in the absence and in increasing concentrations of hematein (10 μM to 100 μM) as indicated. Cellular viability (normalized to DMSO control) was measured after 48 hours using CellTiter-Glo® Luminescent cell viability assay. Data points represent the average of IC50 value of hematein in triplet experiments and bars indicate SD. "*" denotes p < 0.05 when compared with IC50 values of CCL-211 cells and "†" denotes p < 0.05 when compared with IC50 values of WI-38 cells. B. CCL-211 cells were incubated in the absence and presence of 50 μM hematein for 48 hours. Block arrows indicate staining of the nucleus after hematein incubation. Original magnification: × 100.