| Â | GAPDH | NGAL | MMP-9 |
|---|
Number
| 100 | 100 | 100 |
PCR efficiencies
| 2 | 2 | 2 |
Control means
| 19.974 | 29.776 | 28.555 |
Sample means
| 20.077 | 27.364 | 26.889 |
Expression ratios
| Â | 5.716 | 3.408 |
p
values
| Â | 0.001 | 0.001 |
Expression ratios-nn
| 0.931 | 5.322 | 3.173 |
p
values-nn
| 0.709 | 0.001 | 0.001 |
Randomisations
| 1,000 of 1,000 done |
- REST: Relative expression software tool.
- Sample means: Mean CT values for the rectal cancer tissues.
- Control means: Mean CT values for the adjacent normal tissues.
- Expression ratios-nn: Expression ratios not normalised.
-
p values-nn: p values not normalised.
- Randomisations: REST (Relative Expression Software Tool) is a standalone software tool to estimate up and down regulation for gene expression study in real-time PCR by using statistical randomization tests, while taking into account issues of reaction efficiency and reference gene normalisation. The randomization scenario is as follows: "if any perceived variation between samples and controls is due only to chance, then we could randomly swap values between the 2 groups and not see any greater difference than what we see between the initial groups."In our study, the hypothesis test performs 1,000 random reallocations of samples and controls between the groups, and counts the number of times the relative expression on the randomly assigned group is greater than the sample data.
