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Table 3 T50, Tto, Ttd and Ttd – Tto values for known oligonucleotides

From: Quantitation of DNA methylation by melt curve analysis

Oligonucleotide T50 Tto Ttd Ttd – Tto
Unmethylated 79.07 ± 0.06 78.48 ± 0.05 80.60 ± 0.06 2.12 ± 0.07
Partially methylated 80.00 ± 0.26 78.85 ± 0.53 82.39 ± 0.17 3.54 ± 0.47
Fully methylated 82.10 ± 0.00 81.13 ± 0.05 84.29 ± 0.03 3.16 ± 0.15
U/P 79.27 ± 0.21 78.30 ± 0.05 81.47 ± 0.18 3.17 ± 0.15
U/M 80.17 ± 0.15 78.45 ± 0.11 83.36 ± 0.19 4.90 ± 0.21
  1. U/P – unmethylated oligonucleotide spiked with partially methylated oligonucleotide.
  2. U/M – unmethylated oligonucleotide spiked with fully methylated oligonucleotide.
  3. All values are the mean ± standard deviation of 3 replicate reactions.