Figure 4
From: Bone sialoprotein does not interact with pro-gelatinase A (MMP-2) or mediate MMP-2 activation
Zymography analysis of BSP-treated proMMP-2. ProMMP-2 was incubated with (lanes 5–8) or without (lanes 1–4) increasing amounts of BSP for 4 hours at 21°C, and resolved by zymography. ConA cell-activated MMP-2 were used as standards (lanes 9–10). Lane 1, 2 ng proMMP-2; lane 2, 0.5 ng proMMP-2; lane 3, 0.2 ng proMMP-2; lane 4, 0.05 ng proMMP-2; lane 5, 2 ng proMMP-2 + 2 ng BSP; lane 6, 0.5 ng proMMP-2 + 2 ng BSP; lane 7, 0.2 ng proMMP-2 + 2 ng BSP; lane 8, 0.05 ng proMMP-2 + 2 ng BSP; lanes 9 and 10, 0.05 ng conA activated MMP-2.