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Table 1 Antibodies used in this study

From: Paxillin-Y118 phosphorylation contributes to the control of Src-induced anchorage-independent growth by FAK and adhesion

(A) Phospho-specific antibodies
Substrate poY location Mol mass (kDa) Co.; Cat. #a MM/RP/GPb
cortactin 421 80/85 BioS; 44–854 RP
paxillin 31 68 BioS; 44–720 RP
paxillin 118 68 BioS; 44–722 RP
Shc 239/240 46, 52, 66 BioS; 44–830 RP
PKC-δ 311 77 BioS; 44–950 RP
PLC-γ1 783 130 Bios; 44–696 RP
p56Dok-2 351 56, 58 CS; 3911 RP
CAS 410 130 CS; 4011 RP
connexin-43 265 43 SC; sc-17220 GP
p120catenin 228 120 BD; 612536 MM
vinculin 100 130 BioS; 44–1074G RP
vinculin 1065 130 BioS; 44–1078G RP
(B) Antibodies used for IP
Substrate Mol mass (kDa) Co.; Cat. # a MM/RP b  
Sam68 68 SC; sc-333 RP  
Annexin II (H-50) 36(90) SC; sc-9061 RP  
p190Rho-GAP 190 BD; 610149 MM  
Eps8 97 BD; 610143 MM  
Crk (Crk II) 40 BD; 610035 MM  
(C) Antibodies used for total substrate protein level
Substrate Mol mass (kDa) Co.; Cat. # a MM/RP b  
cortactin 80/85 CS; 3502 RP  
paxillin 68 BD; 610551 MM  
Shc 46, 52, 66 CS; 2432 RP  
PKC-δ 77 SC; sc-213 RP  
PLC-γ1 130 CS; 2822 RP  
p56Dok-2 56, 58 CS; 3914 RP  
CAS 130 BD; 610271 MM  
p120catenin 120 BD; 610133 MM  
vinculin 130 Sigma; V-9131 MM  
GAPDH 37 SC; sc-25778 RP  
(D) Epitope-tag antibodies
Epitope tag Co.; Cat. # a MM/RP b   
GST SC; sc-138 MM   
FLAG Sigma; F3040 MM   
GFP Invitrogen; A6455 RP   
  1. a BioS – BioSource; CS – Cell Signaling; SC – Santa Cruz; BD – BD Biosciences
  2. b Abs: RP – Rabbit polyclonal; MM – Mouse monoclonal; GP – Goat polyclonal
  3. * Abs to actinin (Sigma; A5044), CASpoY165 (CS; 4015), CASpoY249 (CS; 4014), p120cateninpoY96 (BD; 612534), p120cateninpoY280 (BD; 612538), ezrinpoY353 (CS; 3144) did not recognize appropriately-sized bands.