Paxillin-Y118 phosphorylation contributes to the control of Src-induced anchorage-independent growth by FAK and adhesion

Table 1 Antibodies used in this study

(A) Phospho-specific antibodies
Substrate	poY location	Mol mass (kDa)	Co.; Cat. #^a	MM/RP/GP^b
cortactin	421	80/85	BioS; 44–854	RP
paxillin	31	68	BioS; 44–720	RP
paxillin	118	68	BioS; 44–722	RP
Shc	239/240	46, 52, 66	BioS; 44–830	RP
PKC-δ	311	77	BioS; 44–950	RP
PLC-γ1	783	130	Bios; 44–696	RP
p56Dok-2	351	56, 58	CS; 3911	RP
CAS	410	130	CS; 4011	RP
connexin-43	265	43	SC; sc-17220	GP
p120catenin	228	120	BD; 612536	MM
vinculin	100	130	BioS; 44–1074G	RP
vinculin	1065	130	BioS; 44–1078G	RP
(B) Antibodies used for IP
Substrate	Mol mass (kDa)	Co.; Cat. # ^a	MM/RP ^b
Sam68	68	SC; sc-333	RP
Annexin II (H-50)	36(90)	SC; sc-9061	RP
p190Rho-GAP	190	BD; 610149	MM
Eps8	97	BD; 610143	MM
Crk (Crk II)	40	BD; 610035	MM
(C) Antibodies used for total substrate protein level
Substrate	Mol mass (kDa)	Co.; Cat. # ^a	MM/RP ^b
cortactin	80/85	CS; 3502	RP
paxillin	68	BD; 610551	MM
Shc	46, 52, 66	CS; 2432	RP
PKC-δ	77	SC; sc-213	RP
PLC-γ1	130	CS; 2822	RP
p56Dok-2	56, 58	CS; 3914	RP
CAS	130	BD; 610271	MM
p120catenin	120	BD; 610133	MM
vinculin	130	Sigma; V-9131	MM
GAPDH	37	SC; sc-25778	RP
(D) Epitope-tag antibodies
Epitope tag	Co.; Cat. # ^a	MM/RP ^b
GST	SC; sc-138	MM
FLAG	Sigma; F3040	MM
GFP	Invitrogen; A6455	RP

^a BioS – BioSource; CS – Cell Signaling; SC – Santa Cruz; BD – BD Biosciences
^b Abs: RP – Rabbit polyclonal; MM – Mouse monoclonal; GP – Goat polyclonal
* Abs to actinin (Sigma; A5044), CAS^poY165 (CS; 4015), CAS^poY249 (CS; 4014), p120catenin^poY96 (BD; 612534), p120catenin^poY280 (BD; 612538), ezrin^poY353 (CS; 3144) did not recognize appropriately-sized bands.

ISSN: 1471-2407