Figure 7
P2X 7 immunoreactivity in mouse normal skin (A), papilloma (C), and skin cancer tissues (E) (Experiment-1); B, D, F are parallel cross sections, respectively, stained by H&E. G. Analysis of P2X7 immunoreactivity compared among paired histologically normal and cancerous tissues. Bars are means (± SD) of levels in tissues of five mice. H. P2X7 protein assays in mouse normal and cancer skin tissues. Lysates fractionated by gel electrophoresis were immunoblotted with the anti P2X7 antibody and membranes were reprobed with the anti GAPDH antibody. Insert in H shows Western immunoblot of lysates of histologically normal and cancerous tissues obtained from the same animal. Similar results were obtained in tissues of two additional mice. Bars show means (± SD) of densitometry results of the P2X7-specific 75 KDa bands in tissues of three mice. I. P2X7 mRNA levels (relative to GAPDH mRNA) (means ± SD) in histologically normal and cancerous tissues obtained from three animals. In G-I data in the normal tissues were normalized in each case to an arbitrary value of 10. * – p < 0.01. AU – arbitrary units.