Figure 14
A. Dependence of the BzATP-induced apoptosis on extracellular calcium (Ca 2+ o ). Cultured mouse wild-type normal keratinocytes were shifted for 10 minutes to medium containing one of the indicated Ca2+ o concentrations. Control (physiological) level of Ca2+ o was 1.2 mM, and levels of Ca2+ o were modulated by adding EGTA. Cells were treated with 100 μM BzATP (or the vehicle, Control), and changes in apoptosis were determined after 8 hours. B. Modulation of BzATP-induced apoptosis (100 μM, 8 hours) in mouse wild-type normal keratinocytes by caspase inhibitors (each added at 50 μM for 8 hours). In A and B changes in apoptosis were determined in terms of solubilized DNA. Values are means (± SD) of 3 experiments. Levels of apoptosis were normalized to an arbitrary value of 2 in non-treated cells. AU – arbitrary units. In A, * – p < 0.01 compared to Ca2+ o 1.2 mM. In B, * – p < 0.01 compared to control.