Comparison of whole body fluorescence imaging and ultrasound imaging to monitor tumor growth over time in individual mice. Whole-body fluorescence and ultrasound images were obtained on a weekly basis for a period of four weeks. Tumor measurements were determined as explained in the legend for Figure 2. A. The graph shows the growth of an orthotopic pancreatic-tumor implant in a single mouse (M1) as assessed by both whole-body fluorescence (FL) and ultrasound (US) imaging. The four corresponding ultrasound (upper row) and color fluorescence (lower row) images are shown below the graph with the calculated tumor sizes (mm2) for each image indicated. B. Graphical depictions of tumor growth over a four week period for an additional four mice (M2 thru M5) with orthotopic pancreatic tumor implants. Bar graphs shown in this figure depict single measurements taken at weekly timepoints. The graphs demonstrate a strong correlation between whole-body fluorescence and ultrasound imaging modalities for determination of tumor size.