Changes of IGFBP5 cellular distribution promotes breast cancer cell proliferation. The cell growth rates for the vector and IGFBP5 transfectants were measured by BrdU incorporation using the BrdU flow kits per the manufacturer's instruction. A. The cells were incubated in 10% FBS medium containing 10 μM BrdU for 2 h followed by immunofluorescence staining with FITC conjugated BrdU antibody. The nuclei were counterstained with DAPI and images were taken at a magnification of 63×. Non-BrdU-treated parental cells were served as a negative control. B. Quantification data from 3 fields of each group. The total cells and the BrdU-positive cells were counted from 3 fields of each group, and the data were graphed as a ratio of BrdU positive cells vs. the total cells. The error bars represents standard deviation from three counts of samples. *, p < 0.05 and **, p < 0.005.C. The geometric mean fluorescence intensity from the same cell population as in A was recorded by FACS. D. The fluorescence intensity shown in B is shown as a bar graph. The fluorescence intensity from the negative control is graphed as zero, and the fluorescence intensity from the rest of the samples is the value after the value of the fluorescence intensity is subtracted from the negative control.