HSP27 was involved in activation of NF-kB pathway in HCC cell lines. Nuclear NF-κB concentration was analyzed by ELISA in HSP27 RNAi, MOCK and MHCC97H cells (A) and in Hep3B, MHCC97L and MHCC97H cells (B). The values represent the relative intensity measured at 450 nm of these different treated cell lysates. The results represent means of triplicates. ** statistical difference from control at P < 0.01.