Figure 7

c-MYC siRNA-mediates inhibition of cisplatin- and etoposide-induced apoptosis (A, B) in DAOY MB cells that is associated with reduced processing of caspase-9 (C). Minor (A) and moderate (B) induction of apoptosis in DAOY V11 cells transfected with siRNAs and exposed to cisplatin and etoposide, respectively. Markedly reduced apoptosis in DAOY M2 cells transfected with c-MYC siRNA compared to control siRNA-transfected cells upon cisplatin and etoposide treatment (A, B). Reduced processing of caspase-9 in c-MYC siRNA-transfected DAOY M2 cells after cisplatin and etoposide treatment as assessed by Western blot analysis (C). Actin was used as a loading control. Values represent the mean absorbance of cytoplasmatic histone-associated DNA fragments (representative of two independent experiments, (n = 3; ± SD)).