Figure 3

Elimination of Jurkat cells is dependent on the CD95 pathway. Jurkat and Jurkat^R cells were cultured in the presence of different sera obtained from control volunteers (C17, C19), women with CIN 1 (D8, D21) and serum from a woman with cervical cancer (O20). After 3 days of incubation at 37°C, the apoptosis induced in each T-cell line was measured by flow cytometry with Annexin-V-Fluos as a marker. Control: Jurkat or Jurkat^R cells grown only with complete RPMI medium. M1 represents the percentage of cells undergoing apoptosis. A total of 10,000 events were measured; analyses were performed using the WinMDI2.8 software. Histograms are representative of separate experiments performed at least twice per condition.