Elimination of Jurkat cells is dependent on the CD95 pathway. Jurkat and JurkatR cells were cultured in the presence of different sera obtained from control volunteers (C17, C19), women with CIN 1 (D8, D21) and serum from a woman with cervical cancer (O20). After 3 days of incubation at 37°C, the apoptosis induced in each T-cell line was measured by flow cytometry with Annexin-V-Fluos as a marker. Control: Jurkat or JurkatR cells grown only with complete RPMI medium. M1 represents the percentage of cells undergoing apoptosis. A total of 10,000 events were measured; analyses were performed using the WinMDI2.8 software. Histograms are representative of separate experiments performed at least twice per condition.