Figure 5
(A) Effect of Ubenimex alone or combined with radiation on s.c. HeLa tumor volumes (mm3) over time (days). Mice were treated with PBS, bestatin, 4 Gy+PBS, and 4 Gy+bestatin, and tumor volumes were monitored over time. Mice were sacrificed on 23rd day. (B) Photographs of tumors on the 23rd day. (C) Tumor weight on the 23rd day. Optimal growth inhibition was observed in the 4 Gy+bestatin group compared with the other groups. (D)TUNEL assay of mouse tumor sections. This method allows the direct detection of DNA fragmentation. The bright cells are apoptotic cells. More apoptotic cells were observed in the combined-treatment group than in the other groups. Errors bar represent the standard error of the mean.