Table 1 Primary antibodies, dilutions, antigen retrieval, and sources of antibodies used in the immunohistochemical study
ANTIBODY | CLONE | DILUTION | ANTIGEN RETRIEVAL | SOURCE/COUNTRY |
|---|---|---|---|---|
D2-40 | D2-40 | 1:100 | No | Signet/USA |
CD31 | JC/70A | 1:50 | Yes | Dako/USA |
P53 | DO7 | 1:400 | Yes | Dako/USA |
ER | 6F-11 | 1:100 | Yes | Novocastra/UK |
PR | PgR 312 | 1:100 | Yes | Novocastra/UK |
Ki67 | MIB-1 | 1:50 | Yes | Immunotech/France |
Her2/neu | CB11 | 1:80 | No | Novocastra/UK |
E-cadherin | NCH-38 | 1:50 | Yes | Dako/USA |
Cytokeratin | AE1/AE3 | 1:100 | Yes | Dako/USA |