Table 2 CT values obtained after MGMT amplification of no methylated DNA (from blood), in vitro methylated DNA (IMD) and no template control (H2O) by MCA-Meth analysis, with and without the adition of the extra incubation step. CT values get reduced (primer dimers do not get amplified) when using the incubation step. No amplification at all is produced in the no template control (H2O), meaning that the CT value of 36.8 corresponded to primer dimer amplification.
Blood | IMD | H2O | |
|---|---|---|---|
CT without step | 35.5 | 38.6 | 36.8 |
CT with step | 34.2 | 38.3 | - |