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Figure 2 | BMC Cancer

Figure 2

From: Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR

Figure 2

MCA-MSP analysis in BLU and MGMT genes for the A172 and SIMA cell lines: The graphs show the unmethylated control peak (blood DNA) in red, the methylated control peak in green, the no template control in blue and the cell line peak, in brown. a) PCR for the unmethylated sequence, BLU gene, A172 cell line: only the blood DNA gives a peak, thus the cell line contains no unmethylated DNA. b) PCR for the methylated sequence, BLU gene, A172 cell line: the methylated DNA and the cell line give peak, thus the A172 cell line is methylated for the BLU gene. c) PCR for the unmethylated sequence, MGMT gene, SIMA cell line: the blood DNA and cell lines give peak, thus the cell line is unmethylated. d) PCR for the methylated sequence, MGMT gene, SIMA cell line: only the methylated DNA gives a peak, thus the cell line is not methylated.

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BMC Cancer

ISSN: 1471-2407

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