Figure 4
Effect of berberine on As 2 O 3 -inhibited invasiveness of C6 glioma and U-87 cells. (A) Cells were incubated with 5μM As2O3 or 10 μM berberine and co-treated with 5 μM and 10 μM berberine for 24 h. Invasion through a layer of Matrigel was determined by a Boyden Chamber method as described in Methods. The invasiveness was quantified and is presented in the graph (B). Control cells remained untreated. The experiments were performed in triplicate and data are presented as means ± SD. * and ** indicate means that are significantly different when compared to the control group of C6 with P < 0.05 and P < 0.01, respectively. # and ## indicate means that are significantly different when compared to the control group of U-87 with P < 0.05 and P < 0.01, respectively.