Table 2 Primers and restriction enzymes used in the RFLP assays.
From: K-rasmutations in sinonasal cancers in relation to wood dust exposure
Exon | Codon | Primers | Sequence 5' to 3' | Restriction enzymes/recognition site | PCR product/cut fragments |
|---|---|---|---|---|---|
1 | 12 | RFLP12F | ACT GAA TAT AAA CTT GTG GTA GTT GGA CCT | BstN I : 5'..CCTGG..3' 3'..GGACC..5' | 157 bp/ N: 114+29+14 M:143+14 |
RFLP1213R | TCA AAG AAT GGT CCT GGA CC | ||||
1 | 13 | RFLP13F | ATA TAA ACT TGT GGT AGT TCC AGC TGG | Van 91 I : 5'.CCA(N)5TGG.3' 3'.GGT(N)5ACC.5' | 152 bp/ N:126+26 M:152 |
RFLP1213R | TCA AAG AAT GGT CCT GGA CC | ||||
2 | 61 | RFLP61F | CTT GGA TAT TCT CGA CAC AGC TGA T | Bcl I: 5'..TGATCA..3' 3'..ACTAGT..5' | 179 bp/ N:155 M: 179 |
RFLP61R | AAC TAT AAT TAC TCC TTA ATG TCA GCT TA |