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Table 2 Primers and restriction enzymes used in the RFLP assays.

From: K-rasmutations in sinonasal cancers in relation to wood dust exposure

Exon Codon Primers Sequence 5' to 3' Restriction enzymes/recognition site PCR product/cut fragments
1 12 RFLP12F ACT GAA TAT AAA CTT GTG GTA GTT GGA CCT BstN I :
5'..CCTGG..3'
3'..GGACC..5'
157 bp/
N: 114+29+14
M:143+14
   RFLP1213R TCA AAG AAT GGT CCT GGA CC   
1 13 RFLP13F ATA TAA ACT TGT GGT AGT TCC AGC TGG Van 91 I :
5'.CCA(N)5TGG.3'
3'.GGT(N)5ACC.5'
152 bp/
N:126+26
M:152
   RFLP1213R TCA AAG AAT GGT CCT GGA CC   
2 61 RFLP61F CTT GGA TAT TCT CGA CAC AGC TGA T Bcl I:
5'..TGATCA..3'
3'..ACTAGT..5'
179 bp/
N:155
M: 179
   RFLP61R AAC TAT AAT TAC TCC TTA ATG TCA GCT TA