Effect of RGZ and 15d-PGJ
on LMM3 cell viability. A. MTS assay was used to determine cell metabolic activity after 24 h-culture in the presence of RGZ (1 and 100 μM) and 15d-PGJ2 (1 and 10 uM) with or without LPS (500 μg/mL). Results are expressed as absorbance (% of control considered 100%) ***p < 0.001 vs. control. B. The growth-kinetics of LMM3 cells in the presence of 10 μM BADGE, 1 and 100 μMRGZ is shown (**p < 0.01, ***p < 0.001 vs. control). C. NO production by LMM3 tumor cells cultured during 24 h with RGZ (1 and 100 μM) or 15d-PGJ2 (1 and 10 μM) was measured in the supernatants using Griess reagent. Experiments were performed with and without LPS. Results are expressed as μM NO2 respect to a control curve of nitrite. A and B show the results of one representative experiment of three independent assays performed by triplicate with similar results. **p < 0.01; ***p < 0.001 vs control.