(a) Frequency of gains and losses in 83 gastric cancers. A green line denotes a copy number gain and a red line denotes a copy number loss. Gains of 7p, 8q, and 20q, and losses of 4q, 17p, and 21 are frequent in gastric adenocarcinoma. (b-left) Design of a mini-array. The mini-array spotted with 50 BAC clones chosen by two analytical methods of the aCGH data from 83 gastric cancers to estimate the node and liver metastases, peritoneal dissemination, depth of tumor invasion, and histological type, 26 clones with frequent or infrequent DCNAs, and 62 reference clones. A total of 138 BAC clones were spotted in triplicate on two discrete parts of a glass slide, and therefore, each slide was used for two specimens. The diameter of each spot is approximately 200 μm. (b-right) A hybridization image for the mini-array. Note the same color image for three spots each in the mini-array. (c) A comparison of aCGH profiles between the screening chip (upper) and the mini-array (lower) for a case of gastric adenocarcinoma. A profile of aCGH in the mini-array basically replicates that in the screening array. The red lines indicate log2 0.25 and -log2 0.25, respectively. The green and red spots indicate the BAC clones with a copy number gain and loss, respectively.