Figure 3

Genewise clustering of SLIT-ROBO genes in HCC cell lines translates into liver tissues. The normalized expression values of individual SLIT-ROBO genes in liver tissue samples (n = 43) were used to establish the hierarchical clustering in Cluster program and the results were visualized as a heatmap by TreeView. Overexpression and underexpression of individual genes relative to Ct average of normal tissues (n = 8) are represented by red and green colors, respectively. Upper single-row heatmap displays the average expression of all six genes across all tissues. The tree at the top of the heatmap represents samplewise clustering, and the tree on the left of the heatmap (pink) represents genewise clustering. T1-35: tumors; N1-8: normal tissues; 0: stage 0 (non-tumor); 1, 2, 3A, 4: stage 1, stage 2, stage 3A, stage 4 HCC, respectively; N: tumor-adjacent normal tissue; W: well-differentiated; M: moderately differentiated; P: poorly differentiated; N/A: not-assigned.