Figure 4

Indirect immunofluorescence detection of H1x and chromogranin A in sections of paraffin wax-embedded samples from pancreas and small intestine. Chromogranin A (CgA) was used as a marker protein for neuroendocrine cells which show scattered location in these tissues. (A) Chromogranin A-positive cells in pancreatic tissue exhibit a relatively high expression of H1x. Scale bar = 10 μm. (B) Cells of a islet of Langerhans show a strong immunoreaction with the anti-H1x antibody. Scale bar = 20 μm. (C) H1x is abundant in chromogranin A-positive cells of small intestine (arrows) compared to the surrounding cells of the glandulae intestinales and also in some cells of the stroma of the villi (arrowheads). Scale bar = 20 μm.