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Table 3 Comparison of fl-OPN ELISA

From: Development of fragment-specific osteopontin antibodies and ELISA for quantification in human metastatic breast cancer

  IBL Assay Designs R&D Quantikine In house
Capture antibody Anti-human OPN (O-17), rabbit polyclonal IgG, recognizes N-terminal 17IPVKQADSGSSEEKQ Mouse monoclonal antibody to OPN, epitope includes 162SVVYGLRSKSK Mouse monoclonal antibody to OPN Mouse monoclonal anti-human OPN to N-fragment
Antigen Untagged rOPN/CHO cells 6x-C-terminal His tagged rOPN/mouse NS0 cells 6x-C-terminal His tagged rOPN/mouse NS0 cells 6x-C-terminal his tagged rOPN/E. coli
Detecting antibody Anti-human OPN (10A16), mouse IgG, Fab'-HRP, recognizes to the right of thrombin cleavag site 170KSKKFRRPDIQYPDATDE Biotinylated monoclonal antibody to OPN. Epitope is located after the thrombin cleavage site, Polyclonal anti-OPN conjugated to HRP Mouse monoclonal anti-human OPN to C-fragment
Detection and system Full-length OPN, HRP-TMB Full-length OPN, biotin-streptavidin-AP Full-length OPN, HRP-TMB Full-length OPN, biotin-streptavidin-HRP
Measur range 5–320 ng/ml 2–32 ng/ml 0.31–20 ng/ml 0.078–10 ng/ml
Sensitivy 5–320 ng/ml 0.110 ng/ml 0.011 ng/ml 0.014 ng/ml
  1. Characteristics of each assay are indicated.