Skip to main content

Advertisement

Figure 3 | BMC Cancer

Figure 3

From: Development of fragment-specific osteopontin antibodies and ELISA for quantification in human metastatic breast cancer

Figure 3

Comparison of OPN ELISA tests. A) Our assay was tested for quantification of recombinant fl-OPN using monoclonal antibodies 2F10 and 1F11-biotin. 2F10 reacts to an epitope on the N-terminal of human OPN and 1F11-biotin reacts with an epitope on the C-terminal side of human OPN. The optical density of the color product is proportional to the quantity of human OPN. The measuring range is from 78 pg/ml to 10 ng/ml. B) Commercially prepared human OPN standards of high (10.1 ng/ml-13.1 ng/ml), medium (5 ng/ml-6.6 ng/ml) or low (1.7 ng/ml-2.3 ng/ml) concentrations were used in four ELISA tests: our in-house test, and those commercially available from Assay Designs, R&D Systems (Quantikine) and IBL. The IBL results are graphed separately because the IBL quantification yielded values that were extremely high compared to the other assays and the standard ranges.

Back to article page