Figure 3
Effects of EVOO phenolics on breast cancer cell proliferation. SKBR3 cells were incubated with various concentrations of EVOO single phenols (left), fractions containing mainly EVOO lignans (middle) and fractions containing mainly EVOO secoiridoids (right) for 4 days. Cell proliferation, measured using a crystal violet assay as described in "Materials and methods", was expressed as % of untreated cells (dashed line = 100% cell proliferation). Results are means (columns) and 95% confidence intervals (bars) of three independent experiments made in triplicate. Statistically significant differences (one-factor ANOVA analysis) between experimental conditions and unsupplemented control cells are shown. All statistical tests were two-sided.